A Multicomponent Butyrylcholinesterase Preparation for Enzyme Inhibition-Based Assay of Organophosphorus Pesticides

A new method of producing butyrylcholinesterase (BChE) preparations, stable in storage and use, has been proposed. The BChE preparation is the enzyme co-immobilized with 0.2 M 5-5′-dithiobis (2-nitrobenzoic acid) starch or gelatin gel. All experimental preparations retain activity for at least 300 d. based on gel show higher but lower sensitivity to toxicants tested this study compared gel-based preparations. proposed integrated detection anti-cholinesterase substances aqueous solutions using immobilized BChE. After additional incubation solution analyte, limits malathion pirimiphos-methyl determined IC20 values were below their maximum allowable concentrations—0.005 µM 0.03 µM, respectively.